规格：50μl, 100μl
价格：¥1250/50μl, ¥2500/100μl
数据表
| UniProt | P42574 |
| Mol Weight | 32kDa |
| Alternative Names | CASP3; Caspase-3; APOPAIN; CPP32; CPP32B; SCA-1; Caspase3; |
| Immunogen | A synthesized peptide derived from human Caspase-3 |
| Storage | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Purification | Affinity-chromatography |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Description | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position. |
Applications
| Reactivity | Human |
| Application | WB,IHC,ICC/IF,IP,FC |
| Recommended dilution | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 |
| Western blot analysis of Caspase-3 in HEK293 cell lysate. |
| Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody. |
| Immunofluorescent analysis of Hela cells, using Caspase-3 Antibody . |
| All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. |
| All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. |
