KYSE150人食管鳞癌细胞 

KYSE 150; KYSE150; Kyse150; KY150；人食管鳞癌细胞

人

女性，49岁

食管鳞癌 

贴壁生长

上皮细胞样

48.5%RPMI-1640＋48.5%F-12+2% FBS＋1% P/S

气相：95%空气+5%二氧化碳；温度：37℃

90%血清+10%DMSO，液氮储存

1

Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice. 

气相：95%空气+5%二氧化碳；温度：37℃

~13.7小时 (PubMed=1728357);  23小时 (PubMed=25984343);

该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。