规格：50µL/100µL
货号：PN0078
价格：1350元/50µL；2200元/100 µL
全称 | programmed cell death 1 |
别名 | CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
交叉反应 | Human, Rat, Mouse, Pig (predicted: Human, Mouse, Rat) |
产品应用 | WB : 1:5000-1:50000 IHC : 1:2000-1:8000 IF : 1:200-1:800 FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
理论分子量 | 32kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1213ug/ml |
免 疫 原 | PD-1/CD279 fusion protein Ag12470 |
亚 型 | IgG |
纯化方法 | Protein A purification |
Uniprot ID | |
缓 冲 液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
保存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20℃storage. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
背景
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellµLar domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regµLation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calcµLated molecµLar weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecµLar mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).
