规格：50µL/100µL
货号：PN0132
价格：1350元/50µL；2200元/100 µL
全称 | PCNA Polyclonal antibody |
别名 | PCNA
|
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, Rat, Human(predicted: Human, Mouse, Rat,Goat ,Chicken,Medaka Embryos,Sheep, Ducks, Rabbit) |
产品应用 | WB：1:5000-1:50000 IHC：1:1500-1:6000 IF-P： 1：50-500 IF/ICC:1:300-1200 IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
理论分子量 | 50kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 550 ug/ml |
免 疫 原 | |
亚 型 | IgG |
纯化方法 | Antigen affinity purification |
Uniprot ID | |
缓 冲 液 | pH: 7.2,Preservative: 0.01% Sodium azide,Constituents: PBS, 40% Glycerol, 0.05% BSA |
保存条件 | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
背景
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimµLatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimµLate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calcµLated molecµLar weight of PCNA is 29 kDa, but modified PCNA is 36kDa
